23S rRNA (adenine2503-C2,C8)-dimethyltransferase

23S rRNA (adenine²⁵⁰³-C²,C⁸)-dimethyltransferase (EC 2.1.1.194, Cfr, Cfr methyltransferase, Cfr rRNA methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:23S rRNA (adenine²⁵⁰³-C²,C⁸)-dimethyltransferase.^[1]^[2]^[3] This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + adenine²⁵⁰³ in 23S rRNA

\rightleftharpoons

2 S-adenosyl-L-homocysteine + 2,8-dimethyladenine²⁵⁰³ in 23S rRNA

This enzyme contains an [4Fe-S] cluster.

References

↑ Yan, F.; LaMarre, J.M.; Röhrich, R.; Wiesner, J.; Jomaa, H.; Mankin, A.S.; Fujimori, D.G. (2010). "RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA". J. Am. Chem. Soc. 132 (11): 3953–3964. doi:10.1021/ja910850y. PMC 2859901. PMID 20184321.
↑ Giessing, A.M.; Jensen, S.S.; Rasmussen, A.; Hansen, L.H.; Gondela, A.; Long, K.; Vester, B.; Kirpekar, F. (2009). "Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria". RNA. 15 (2): 327–336. doi:10.1261/rna.1371409. PMC 2648713. PMID 19144912.
↑ Kaminska, K.H.; Purta, E.; Hansen, L.H.; Bujnicki, J.M.; Vester, B.; Long, K.S. (2010). "Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria". Nucleic Acids Res. 38 (5): 1652–1663. doi:10.1093/nar/gkp1142. PMC 2836569. PMID 20007606.

External links

23S rRNA (adenine2503-C2,C8)-dimethyltransferase at the US National Library of Medicine Medical Subject Headings (MeSH)

Transferase: one carbon transferases (EC 2.1)

2.1.1: Methyl-

N-	Histamine N-methyltransferase Phenylethanolamine N-methyltransferase Amine N-methyltransferase Phosphatidylethanolamine N-methyltransferase

O-	5-hydroxyindole-O-methyltransferase/Acetylserotonin O-methyltransferase Catechol-O-methyl transferase

Homocysteine	Betaine-homocysteine methyltransferase Homocysteine methyltransferase Methionine synthase

Other	Phosphatidyl ethanolamine methyltransferase DNMT3B Histone methyltransferase Thymidylate synthase DNA methyltransferase Thiopurine methyltransferase

2.1.2: Hydroxymethyl-,
Formyl- and Related

Hydroxymethyltransferase	Serine hydroxymethyltransferase 3-methyl-2-oxobutanoate hydroxymethyltransferase

Formyltransferase	Phosphoribosylglycinamide formyltransferase Inosine monophosphate synthase

Other	Glutamate formimidoyltransferase Aminomethyltransferase

2.1.3: Carboxy-
and Carbamoyl

Carboxy	methylmalonyl-CoA carboxytransferase

Carbamoyl	Aspartate carbamoyltransferase Ornithine carbamoyltransferase Oxamate carbamoyltransferase Putrescine carbamoyltransferase 3-hydroxymethylcephem carbamoyltransferase Lysine carbamoyltransferase N-acetylornithine carbamoyltransferase

2.1.4: Amidine

Arginine:glycine amidinotransferase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list)

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