Chorionic villus sampling

Chorionic villus sampling
Intervention

Micrograph showing chorionic villi - the tissue that is collected in CVS. H&E stain.
ICD-10-PCS 16603-00
ICD-9-CM 75.33
MeSH D015193
MedlinePlus 003406

Chorionic villus sampling (CVS), sometimes called "chorionic villous sampling" (as "villous" is the adjectival form of the word "villus"),[1] is a form of prenatal diagnosis to determine chromosomal or genetic disorders in the fetus. It entails sampling of the chorionic villus (placental tissue) and testing it for chromosomal abnormalities, usually with FISH or PCR. CVS usually takes place at 10–12 weeks' gestation, earlier than amniocentesis or percutaneous umbilical cord blood sampling. It is the preferred technique before 15 weeks.[2]

CVS was performed for the first time by Italian biologist Giuseppe Simoni, scientific director of Biocell Center, in 1983.[3]

Use as early as 8 weeks in special circumstances has been described.[4]

It can be performed in a transcervical or transabdominal manner.[5]

Although this procedure is mostly associated with testing for Down Syndrome, overall, CVS can detect more than 200 disorders.[6]

Indications

Possible reasons for having a CVS can include:

Risks

The risk of miscarriage in CVS is estimated to be about 1-2%.[8] Apart from a risk of miscarriage, there is a risk of infection and amniotic fluid leakage. The resulting amniotic fluid leak can develop into a condition known as oligohydramnios, which is low amniotic fluid level. If the resulting oligohydramnios is not treated and the amniotic fluid continues to leak it can result in the baby developing hypoplastic lungs (underdeveloped lungs).

Additionally, there is also mild risk of Limb Reduction Defects associated with CVS, with the risk being higher the earlier the procedure is carried.[9]

It is important after having CVS that the obstetrician follow the patient closely to ensure the patient does not develop infection.

Chorionic villi and stem cells

Recent studies have discovered that chorionic villi can be a rich source of fetal stem cells, multipotent mesenchymal stem cells[10][11][12]

A potential benefit of using fetal stem cells over those obtained from embryos is that they side-step ethical concerns among anti-abortion activists by obtaining pluripotent lines of undifferentiated cells without harm to a fetus or destruction of an embryo. These stem cells would also, if used to treat the same individual they came from, sidestep the donor/recipient issue which has so far stymied all attempts to use donor-derived stem cells in therapies.

Artificial heart valves, working tracheas, as well as muscle, fat, bone, heart, neural and liver cells have all been engineered through use of fetal stem cells [13]

The first fetal stem cells bank in US is active in Boston, Massachusetts.[14][15][16][17]

Limitations

A small percentage (1-2%) of pregnancies have confined placental mosaicism, where some but not all of the placental cells tested in the CVS are abnormal, even though the pregnancy is unaffected.[18] Cells from the mother can be mixed with the placental cells obtained from the CVS procedure. Occasionally if these maternal cells are not completely separated from the placental sample, this can lead to discrepancies with the results. This phenomenon is called Maternal Cell Contamination (MCC).[18] CVS cannot detect all birth defects. It is used for testing chromosomal abnormalities or other specific genetic disorders only if there is family history or other reason to test.

See also

References

  1. ↑ A PubMed search yields 168 papers using chorionic villous as of June 15, 2011.
  2. ↑ Alfirevic Z, von Dadelszen P (2003). Alfirevic, Zarko, ed. "Instruments for chorionic villus sampling for prenatal diagnosis". Cochrane Database Syst Rev (1): CD000114. doi:10.1002/14651858.CD000114. PMID 12535386.
  3. ↑ Brambati, B.; Simoni, G. (1983). "Diagnosis of fetal trisomy 21 in first trimester". The Lancet. 1 (8324): 586. doi:10.1016/S0140-6736(83)92831-3. PMID 6131275.
  4. ↑ Wapner, Ronald J.; Evans, Mark I.; Davis, George; Weinblatt, Vivian; Moyer, Sue; Krivchenia, Eric L.; Jackson, Laird G. (2002). "Procedural risks versus theology: Chorionic villus sampling for Orthodox Jews at less than 8 weeks' gestation". American Journal of Obstetrics and Gynecology. 186 (6): 1133–6. doi:10.1067/mob.2002.122983. PMID 12066086.
  5. ↑ Alfirevic Z, Sundberg K, Brigham S (2003). Alfirevic, Zarko, ed. "Amniocentesis and chorionic villus sampling for prenatal diagnosis". Cochrane Database Syst Rev (3): CD003252. doi:10.1002/14651858.CD003252. PMID 12917956.
  6. ↑ MedlinePlus Encyclopedia Chorionic villus sampling
  7. ↑ Incidence of Down syndrome | Pregnancy Signs Blog
  8. ↑ "Chorionic villus sampling - Risks". NHS Choices. Retrieved 2016-05-24. Page last reviewed: 06/08/2015
  9. ↑ "Chorionic villus sampling and amniocentesis: recommendations for prenatal counseling. Centers for Disease Control and Prevention". MMWR. 44 (RR-9): 1–12. 1995. PMID 7565548.
  10. ↑ Weiss, Rick (2007-01-08). "Scientists See Potential In Amniotic Stem Cells". The Washington Post. Retrieved 2010-04-23.
  11. ↑ De Coppi, Paolo; Bartsch, Georg; Siddiqui, M Minhaj; Xu, Tao; Santos, Cesar C; Perin, Laura; Mostoslavsky, Gustavo; Serre, Angéline C; Snyder, Evan Y; Yoo, James J; Furth, Mark E; Soker, Shay; Atala, Anthony (2007). "Isolation of amniotic stem cell lines with potential for therapy". Nature Biotechnology. 25 (1): 100–6. doi:10.1038/nbt1274. PMID 17206138.
  12. ↑ "Stem Cells – BiocellCenter". Archived from the original on 11 January 2010. Retrieved 2010-01-11.
  13. ↑ "Stem cells scientific updates – BiocellCenter". Archived from the original on 11 January 2010. Retrieved 2010-01-11.
  14. ↑ "European Biotech Company Biocell Center Opens First united state Facility for Preservation of Amniotic Stem Cells in Medford, Massachusetts | Reuters". 2009-10-22. Archived from the original on October 30, 2009. Retrieved 2010-01-11.
  15. ↑ "Europe's Biocell Center opens Medford office – Daily Business Update – The Boston Globe". 2009-10-22. Archived from the original on 12 January 2010. Retrieved 2010-01-11.
  16. ↑ "The Ticker - BostonHerald.com". Retrieved 2010-01-11.
  17. ↑ "Biocell partner with largest New England's hospital group to preserve amniotic stem cell". Archived from the original on 14 March 2010. Retrieved 2010-03-10.
  18. 1 2 Wapner, Ronald J. (2005). "Invasive Prenatal Diagnostic Techniques". Seminars in Perinatology. 29 (6): 401–4. doi:10.1053/j.semperi.2006.01.003. PMID 16533654.
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