EIF2C1

AGO1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1SI2, 1SI3, 4KRE, 4KRF, 4KXT
Identifiers
Aliases	AGO1, EIF2C, EIF2C1, GERP95, Q99, hAgo1, argonaute 1, RISC catalytic component
External IDs	MGI: 2446630 HomoloGene: 81826 GeneCards: AGO1
Gene ontology Molecular function • RNA polymerase II core binding • core promoter binding • miRNA binding • endoribonuclease activity • RNA polymerase II core promoter proximal region sequence-specific DNA binding • protein binding • single-stranded RNA binding • RNA binding • nucleic acid binding • transcription regulatory region DNA binding • double-stranded RNA binding • poly(A) RNA binding Cellular component • cytoplasm • polysome • cytosol • RISC complex • cytoplasmic mRNA processing body • intracellular ribonucleoprotein complex • micro-ribonucleoprotein complex • nucleoplasm • RISC-loading complex • nucleus Biological process • mRNA cleavage involved in gene silencing by siRNA • miRNA loading onto RISC involved in gene silencing by miRNA • regulation of transcription, DNA-templated • transcription, DNA-templated • pre-miRNA processing • nuclear-transcribed mRNA catabolic process • positive regulation of gene expression • posttranscriptional gene silencing by RNA • miRNA mediated inhibition of translation • RNA secondary structure unwinding • mRNA cleavage involved in gene silencing by miRNA • miRNA metabolic process • gene silencing by RNA • regulation of translation • phosphatidylinositol-mediated signaling • positive regulation of transcription from RNA polymerase II promoter • production of miRNAs involved in gene silencing by miRNA • positive regulation of NIK/NF-kappaB signaling • Wnt signaling pathway, calcium modulating pathway Sources:Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	26523	236511
Ensembl	ENSG00000092847	ENSMUSG00000041530
UniProt	Q9UL18	Q8CJG1
RefSeq (mRNA)	NM_012199 NM_001317122 NM_001317123	NM_153403 NM_001317173 NM_001317174
RefSeq (protein)	NP_036331.1 NP_001304051.1 NP_001304052.1	NP_700452.2 NP_001304102.1
Location (UCSC)	Chr 1: 35.87 – 35.93 Mb	Chr 4: 126.44 – 126.47 Mb
PubMed search	[1]	[2]
Wikidata

Protein argonaute-1 is a protein that in humans is encoded by the EIF2C1 gene.^[3][4][5]

Function

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4.^[5]

Model organisms

Model organisms have been used in the study of EIF2C1 function. A conditional knockout mouse line, called Eif2c1^{tm1a(KOMP)Wtsi[10][11]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.^[12][13][14]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.^[8][15] Twenty two tests were carried out on mutant mice and two significant abnormalities were observed: homozygous mutants were subviable and females also had decreased circulating aspartate transaminase levels. ^[8]

References

Further reading

PDB gallery
1si2: Crystal structure of the PAZ domain of human eIF2c1 in complex with a 9-mer siRNA-like duplex of deoxynucleotide overhang  1si3: Crystal structure of the PAZ domain of human eIF2c1 in complex with a 9-mer siRNA-like duplex