Microsatellite enrichment

Microsatellite enrichment is a method in molecular biology used for enriching the amount of microsatellite sequences in a DNA sample. This can be achieved by designing oligonucleotide probes that hybridize with the repeats in the microsatellites and then pull out the probe/microsatellite complexes from the solution.[1] This has been shown to be a cost-effective method to sample the genetic diversity in non-model organisms.[2]

References

  1. ↑ Kaukinen KH, Supernault KJ, Miller KM (2004). "Enrichment of tetranucleotide microsatellite loci from invertebrate species". Journal of Shellfish Research. 23 (2): 621.
  2. ↑ Jennings, TN; Knaus, BJ; Mullins, TD; Haig, SM; Cronn, RC (2011-06-16). "Multiplexed microsatellite recovery using massively parallel sequencing.". Molecular ecology resources. 11 (6): 1060–7. doi:10.1111/j.1755-0998.2011.03033.x. PMID 21676207.


This article is issued from Wikipedia - version of the 6/3/2016. The text is available under the Creative Commons Attribution/Share Alike but additional terms may apply for the media files.