NS0 cell

NS0 cells are a model cell line derived from the nonsecreting murine myeloma used in biomedical research and commercially in the production of therapeutic proteins.[1] The cell line is a cholesterol-dependent cell line that was generated from a subline of NSI/1 which produced only the light chain but no heavy chain.[2][3]

Cell line development

Development of murine neoplasms started with work with the BALB/c mice to isolate the IgG1 secreting MOPC21 tumor. From this tumor, the P3K cells were isolated and developed into two cell lines, 289-16 and P3-X63. The 289-16 cell line secreted only light chain and no heavy chain and was renamed NSI/1. Clones from that cell line were isolated and a nonsecreting cell line was identified and was named NS0/1.[2][4]

Uses in biotechnology

As myeloma cells, they are naturally antibody producing suspension cells. Gene amplication is typically performed using GS-transfected NS0 cells to select for producing cell lines.[5] The GS-NS0 is a heterologous mammalian expression system that allows for the rapid expression of recombinant proteins. Several therapeutic antibody products are produced using the NS0 cell line including daclizumab and eculizumab.[6]

References

  1. ↑ Barnes, LM; Bentley, CM; Dickson, AJ (February 2000). "Advances in animal cell recombinant protein production: GS-NS0 expression system.". Cytotechnology. 32 (2): 109–23. doi:10.1023/A:1008170710003. PMID 19002973.
  2. 1 2 M. Barnes, Louise; Bentley, Catherine M.; Dickson, Alan J. (2000). "Advances in animal cell recombinant protein production: GS-NS0 expression system". Cytotechnology. 32 (2): 109–123. doi:10.1023/A:1008170710003. PMID 19002973.
  3. ↑ Sato, JD; Cao, HT; Kayada, Y; Cabot, MC; Sato, GH; Okamoto, T; Welsh, CJ (December 1988). "Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium.". In vitro cellular & developmental biology : journal of the Tissue Culture Association. 24 (12): 1223–8. doi:10.1007/bf02624194. PMID 3209588.
  4. ↑ Galfrè, G; Milstein, C (1981). "Preparation of monoclonal antibodies: strategies and procedures.". Methods in enzymology. 73 (Pt B): 3–46. PMID 7300683.
  5. ↑ Barnes, Louise M.; Bentley, Catherine M.; Dickson, Alan J. (20 May 2001). "Characterization of the stability of recombinant protein production in the GS-NS0 expression system". Biotechnology and Bioengineering. 73 (4): 261–270. doi:10.1002/bit.1059.
  6. ↑ Wurm, Florian M (November 2004). "Production of recombinant protein therapeutics in cultivated mammalian cells". Nature Biotechnology. 22 (11): 1393–1398. doi:10.1038/nbt1026.

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