SETMAR
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Histone-lysine N-methyltransferase SETMAR is an enzyme that in humans is encoded by the SETMAR gene.[2][3]
Model organisms
| Characteristic | Phenotype |
|---|---|
| Homozygote viability | Normal |
| Homozygous Fertility | Normal |
| Body weight | Normal |
| Anxiety | Normal |
| Neurological assessment | Normal |
| Grip strength | Normal |
| Hot plate | Normal |
| Dysmorphology | Normal |
| Indirect calorimetry | Normal |
| Glucose tolerance test | Normal |
| Auditory brainstem response | Normal |
| DEXA | Normal |
| Radiography | Normal |
| Body temperature | Normal |
| Eye morphology | Abnormal[4] |
| Clinical chemistry | Normal |
| Plasma immunoglobulins | Normal |
| Haematology | Normal |
| Peripheral blood lymphocytes | Abnormal[5] |
| Micronucleus test | Normal |
| Heart weight | Normal |
| Skin Histopathology | Normal |
| Brain histopathology | Normal |
| Salmonella infection | Normal[6] |
| Citrobacter infection | Normal[7] |
| All tests and analysis from[8][9] |
Model organisms have been used in the study of SETMAR function. A conditional knockout mouse line, called Setmartm1a(EUCOMM)Wtsi[10][11] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[12][13][14]
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[8][15] Twenty five tests were carried out on mutant mice and two significant abnormalities were observed.[8] Homozygous mutant animals of both sex had abnormal retinal pigmentation and morphology, while males also had atypical peripheral blood lymphocyte parameters.[8]
References
- ↑ "Human PubMed Reference:".
- ↑ Robertson HM; Zumpano KL (Feb 1998). "Molecular evolution of an ancient mariner transposon, Hsmar1, in the human genome". Gene. 205 (1-2): 203–17. doi:10.1016/S0378-1119(97)00472-1. PMID 9461395.
- ↑ "Entrez Gene: SETMAR SET domain and mariner transposase fusion gene".
- ↑ "Eye morphology data for Setmar". Wellcome Trust Sanger Institute.
- ↑ "Peripheral blood lymphocytes data for Setmar". Wellcome Trust Sanger Institute.
- ↑ "Salmonella infection data for Setmar". Wellcome Trust Sanger Institute.
- ↑ "Citrobacter infection data for Setmar". Wellcome Trust Sanger Institute.
- 1 2 3 4 Gerdin, AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
- ↑ Mouse Resources Portal, Wellcome Trust Sanger Institute.
- ↑ "International Knockout Mouse Consortium".
- ↑ "Mouse Genome Informatics".
- ↑ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410
. PMID 21677750. - ↑ Dolgin, Elie (2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ↑ International Mouse Knockout Consortium; Collins, FS; Rossant, J; Wurst, W (2007). "A Mouse for All Reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
- ↑ van der Weyden L; White JK; Adams DJ; Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
Further reading
- Berry R, Stevens TJ, Walter NA, et al. (1995). "Gene-based sequence-tagged-sites (STSs) as the basis for a human gene map.". Nat. Genet. 10 (4): 415–23. doi:10.1038/ng0895-415. PMID 7670491.
- Maruyama K; Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene. 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
- Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction.". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
- Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing.". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
- Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene. 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
- Lee SH, Oshige M, Durant ST, et al. (2006). "The SET domain protein Metnase mediates foreign DNA integration and links integration to nonhomologous end-joining repair.". Proc. Natl. Acad. Sci. U.S.A. 102 (50): 18075–80. Bibcode:2005PNAS..10218075L. doi:10.1073/pnas.0503676102. PMC 1312370. PMID 16332963.
- Cordaux R; Udit S; Batzer MA; Feschotte C (2006). "Birth of a chimeric primate gene by capture of the transposase gene from a mobile element.". Proc. Natl. Acad. Sci. U.S.A. 103 (21): 8101–6. Bibcode:2006PNAS..103.8101C. doi:10.1073/pnas.0601161103. PMC 1472436. PMID 16672366.
- Keravala A, Liu D, Lechman ER, et al. (2007). "Hyperactive Himar1 transposase mediates transposition in cell culture and enhances gene expression in vivo.". Hum. Gene Ther. 17 (10): 1006–18. doi:10.1089/hum.2006.17.1006. PMID 16989604.
- Liu D, Bischerour J, Siddique A, et al. (2007). "The human SETMAR protein preserves most of the activities of the ancestral Hsmar1 transposase.". Mol. Cell. Biol. 27 (3): 1125–32. doi:10.1128/MCB.01899-06. PMC 1800679. PMID 17130240.
- Miskey C, Papp B, Mátés L, et al. (2007). "The ancient mariner sails again: transposition of the human Hsmar1 element by a reconstructed transposase and activities of the SETMAR protein on transposon ends.". Mol. Cell. Biol. 27 (12): 4589–600. doi:10.1128/MCB.02027-06. PMC 1900042. PMID 17403897.
- Roman Y, Oshige M, Lee YJ, et al. (2007). "Biochemical characterization of a SET and transposase fusion protein, Metnase: its DNA binding and DNA cleavage activity.". Biochemistry. 46 (40): 11369–76. doi:10.1021/bi7005477. PMC 3374406. PMID 17877369.
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