RNA spike-in

An RNA spike-in is an RNA transcript used to calibrate measurements in a DNA microarray experiment. Each spike-in is designed to hybridize with a specific control probe on the target array. Manufacturers of commercially available microarrays typically offer companion RNA spike-ins "kits".[1][2][3][4]

Known amounts of RNA spike-ins are mixed with the experiment sample during preparation. Subsequently the measured degree of hybridization between the spike-ins and the control probes is used to normalize the hybridization measurements of the sample RNA.

See also

References

  1. ↑ Yang IV (2006). "Use of external controls in microarray experiments.". Methods Enzymol. 411: 50–63. doi:10.1016/S0076-6879(06)11004-6. PMID 16939785.
  2. ↑ Fardin P, Moretti S, Biasotti B, Ricciardi A, Bonassi S, Varesio L (2007). "Normalization of low-density microarray using external spike-in controls: analysis of macrophage cell lines expression profile.". BMC Genomics. 8: 17. doi:10.1186/1471-2164-8-17. PMC 1797020Freely accessible. PMID 17229315.
  3. ↑ Wilkes T, Laux H, Foy CA (2007). "Microarray data quality - review of current developments.". OMICS. 11 (1): 1–13. doi:10.1089/omi.2006.0001. PMID 17411392.
  4. ↑ Schuster EF, Blanc E, Partridge L, Thornton JM (2007). "Estimation and correction of non-specific binding in a large-scale spike-in experiment.". Genome Biol. 8 (6): R126. doi:10.1186/gb-2007-8-6-r126. PMC 2394775Freely accessible. PMID 17594493.


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