Toluene dioxygenase
| toluene dioxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 1.14.12.11 | ||||||||
| CAS number | 120038-36-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / EGO | ||||||||
| |||||||||
In enzymology, a toluene dioxygenase (EC 1.14.12.11) is an enzyme that catalyzes the chemical reaction
- toluene + NADH + H+ + O2 (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
The 4 substrates of this enzyme are toluene, NADH, H+, and O2, whereas its two products are (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol and NAD+.
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of two atoms o oxygen into the other donor. The systematic name of this enzyme class is toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating). This enzyme is also called toluene 2,3-dioxygenase. This enzyme participates in toluene and xylene degradation.
References
- Renganathan V (1989). "Possible involvement of toluene-2,3-dioxygenase in defluorination of 3-fluoro-substituted benzenes by toluene-degrading Pseudomonas sp strain T-12". Appl. Exp. Microbiol. 55: 330–334.
- Subramanian V, Liu TN, Yeh WK, Gibson DT (1979). "Toluene dioxygenase: purification of an iron-sulfur protein by affinity chromatography". Biochem. Biophys. Res. Commun. 91 (3): 1131–9. doi:10.1016/0006-291X(79)91998-3. PMID 526270.