Tyrosine—tRNA ligase
| tyrosine-tRNA ligase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 6.1.1.1 | ||||||||
| CAS number | 9023-45-4 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / EGO | ||||||||
| |||||||||
In enzymology, a tyrosine-tRNA ligase (EC 6.1.1.1) is an enzyme that catalyzes the chemical reaction
- ATP + L-tyrosine + RNA AMP + diphosphate + L-tyrosyl-tRNATyr
The 3 substrates of this enzyme are ATP, L-tyrosine, and RNA, whereas its 3 products are AMP, diphosphate, and L-tyrosyl-tRNA(Tyr).
This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-tyrosine:tRNATyr ligase (AMP-forming). This enzyme participates in phenylalanine, tyrosine and tryptophan biosynthesis and aminoacyl-trna biosynthesis.
Structural studies
As of late 2007, 34 structures have been solved for this class of enzymes, with PDB accession codes 1H3E, 1J1U, 1JH3, 1JII, 1JIJ, 1JIK, 1JIL, 1N3L, 1NTG, 1Q11, 1TYA, 1TYB, 1TYC, 1TYD, 1U7D, 1U7X, 1VBM, 1VBN, 1WQ3, 1WQ4, 1X8X, 1Y42, 1ZH0, 1ZH6, 2AG6, 2CYA, 2CYB, 2CYC, 2DLC, 2HGZ, 2J5B, 2TS1, 3TS1, and 4TS1.
References
- ALLEN EH, GLASSMAN E, SCHWEET RS (1960). "Incorporation of amino acids into ribonucleic acid. I. The role of activating enzymes". J. Biol. Chem. 235: 1061–7. PMID 13792726.
- Cowles JR, Key JL (1972). "Demonstration of two tyrosyl-tRNA synthetases of pea roots". Biochim. Biophys. Acta. 281 (1): 33–44. doi:10.1016/0005-2787(72)90185-2. PMID 4563531.
- HOLLEY RW, BRUNNGRABER EF, SAAD F, WILLIAMS HH (1961). "Partial purification of the threonine- and tyrosine-activating enzymes from rat liver, and the effect of patassium ions on the activity of the tyrosine enzyme". J. Biol. Chem. 236: 197–9. PMID 13715350.
- Schweet RS; Allen EH (1958). "Purification and properties of tyrosine-activating enzyme of hog pancreas". J. Biol. Chem. 233: 1104–1108. PMID 13598741.
- Brick P, Bhat TN, Blow DM (1989). "Structure of tyrosyl-tRNA synthetase refined at 2.3 A resolution Interaction of the enzyme with the tyrosyl adenylate intermediate". J. Mol. Biol. 208 (1): 83–98. doi:10.1016/0022-2836(89)90090-9. PMID 2504923.